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3D printed Chitosan Composite Scaffold for Chondrocytes differentiation

Author(s):

Nitin Sahai*, Manashjit Gogoi and Ravi Prakash Tewari   Pages 1 - 13 ( 13 )

Abstract:


3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration. In the present study, different compositions of chitosan-gelatin-alginate composite scaffolds with controlled porosity and architectures were 3D printed. To obtain the desired scaffold, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer. Stereolithography (STL) files of 3D models for porous chitosan composite were created using computeraided design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min. The prepared composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD), scanning electron microscopy SEM, swelling property, mechanical testing, porosity, etc. In-vitro cell culture study was observed on 3D printed chitosan, gelatin, and alginate hydrogel scaffolds. The prepared scaffolds were highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation. The 3D printed chitosan composite scaffolds supported the differentiation of chondrocytes. The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.

Keywords:

Chitosan, Hydrogel, 3D Printing, Cell Culture, Chondrocytes, Tissue Engineering.

Affiliation:

Department of Biomedical Engineering, North-Eastern Hill University, Shillong-793022, Meghalaya, Department of Biomedical Engineering, North-Eastern Hill University, Shillong-793022, Meghalaya, Department of Applied Mechanics, Motilal Nehru National Institute of Technology, Allahabad, Prayagraj-211004, Uttar Pradesh



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